Localized in Situ Hydrogel-Mediated Protein Digestion and Extraction Technique for on-Tissue Analysis
نویسندگان
چکیده
منابع مشابه
Synthesis and Characterization of an Enzyme Mediated in situ Forming Hydrogel Based on Gum Tragacanth for Biomedical Applications
Background: The excellent biocompatibility, biodegradability and biological properties of the hydrogels, fabricated using natural polymers, especially polysaccharides, are very advantageous for biomedical applications. Gum tragacanth (GT) is a heterogeneous highly branched anionic polysaccharide, which has been used extensively in food and pharmaceutical industries. Despite, its desirable prop...
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چکیده ندارد.
15 صفحه اولRealization of on-tissue protein identification by highly efficient in situ digestion with graphene-immobilized trypsin for MALDI imaging analysis.
A novel implementation of in situ protein digestion supported by a graphene oxide-immobilized enzyme reactor (GO-IMER) in the MALDI imaging mass spectrometry (IMS) workflow is reported, which enables the simultaneous diagnostic identity and distribution attributes of the proteome on tissue.
متن کاملsynthesis and characterization of an enzyme mediated in situ forming hydrogel based on gum tragacanth for biomedical applications
background: the excellent biocompatibility, biodegradability and biological properties of the hydrogels, fabricated using natural polymers, especially polysaccharides, are very advantageous for biomedical applications. gum tragacanth (gt) is a heterogeneous highly branched anionic polysaccharide, which has been used extensively in food and pharmaceutical industries. despite, its desirable prop...
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The use of proteomics approaches is a powerful tool in food science in terms of process optimization and monitoring, quality, traceability, safety, and nutritional assessment (Pedreschi et al. 2010 ) . Proteins, together with peptides, are one of the major groups of food components, and they are found in many different organisms of both vegetal and animal origin. Peptides are also obtained duri...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2013
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac3031493